Gene Bridges GmbH,成立于2000年,是一家從歐洲分子生物學實驗室(EMBL)分離出來的獨立經營的公司,主要經營其產品Red/ET重組技術的試劑盒。 Red/ET重組是新近出現的一種基于λ噬菌體Red操縱子(Redα/Redβ/Redγ)和Rac噬菌體RecE/RecT重組系統的DNA工程技術。 通過該技術可以簡單、快速地對任意大的DNA分子進行插入、敲除、突變等多種修飾,而且還可對 長達80kb的DNa片段進行亞克隆。由于重組反應的整個過程都是在大腸桿菌細胞內部完成,因此不存在堿基突變危險。該技術已被廣泛地用于基因組DNA, 如細菌人工染色體、大腸桿菌染色體等的遺傳修飾研究以及基因工程藥物的研究和開發中。 Cat# | Name | Size | Price | K004 | Quick & Easy Red/ET Conditional Knock Out Kit - FRT (need license to buy) | Kit | 15249 | K005 | Quick & Easy Red/ET Conditional Knock Out Kit - loxP (need license to buy) | Kit | 15249 | K006 | Quick & Easy Red/ET E. coli Gene Deletion Kit (need license to buy) | Kit | 13039 | A008 | Red/ET Ampicillin Selection Cassette, FRT-flanked (FRT-amp-FRT) | 50ng/ul,20ul | 6077.5 | A009 | Red/ET Ampicillin Selection Cassette, loxP flanked (loxP-amp-loxP) | 50ng/ul,20ul | 6077.5 | K003 | Red/ET BAC Subcloning Kit (need license to buy) | Kit | 13260 | A006 | Red/ET Chloramphenicol Selection Cassette, FRT-flanked (FRT-cm-FRT) | 50ng/ul,20ul | 6077.5 | A007 | Red/ET Chloramphenicol Selection Cassette, loxP-flanked (loxP-cm-loxP) | 50ng/ul,20ul | 6077.5 | K002 | Red/ET Counter Selection BAC Modification Kit (need license to buy) | Kit | 17459 | A112 | Red/ET Cre Expression Plasmid:705-Cre, cm-selection | 0.2ug/ul,20ul | 6077.5 | A113 | Red/ET Cre Expression Plasmid:706-Cre, tet-selection | 0.2ug/ul,20ul | 6077.5 | A201 | Red/ET Enhanced Eukaryotic FLP Expression Plasmid: pCAGGS-FLPe (Academic price, requires MTA) | 0.2ug/ul,20ul | 8287.5 | A202 | Red/ET Enhanced Eukaryotic FLP Expression Plasmid: pCAGGS-FLPe (Industry price, requires MTA) | 0.2ug/ul,20ul | 71825 | A104 | Red/ET Enhanced Flp Expression Plasmid:707-FLPe, tet-selection | 0.2ug/ul,20ul | 6077.5 | A105 | Red/ET Enhanced Flp Expression Plasmid:708-FLPe, cm-selection | 0.2ug/ul,20ul | 6077.5 | A010 | Red/ET Hygromycin Selection Cassette, FRT-flanked, Prokaryotic and Eukaryotic (FRT-PGK-gb2-hygro-FRT) | 50ng/ul,20ul | 6077.5 | A011 | Red/ET Hygromycin Selection Cassette, loxP-flanked, Prokaryotic and Eukaryotic (loxP-PGK-gb2-hygro-loxP) | 50ng/ul,20ul | 6077.5 | A005 | Red/ET Neomycin Selection Cassette plus loxP site 2nd version, FRT flanked, Prokaryotic and Eukaryotic (loxP-FRT-PGK-gb2-neo-FRT) | 50ng/ul,20ul | 6077.5 | A004 | Red/ET Neomycin Selection Cassette plus loxP site, FRT flanked, Prokaryotic and Eukaryotic (FRT-PGK-gb2-neo-FRT-loxP) | 50ng/ul,20ul | 6077.5 | A002 | Red/ET Neomycin Selection Cassette, FRT-flanked, Prokaryotic and Eukaryotic (FRT-PGK-gb2-neo-FRT) | 50ng/ul,20ul | 6077.5 | A003 | Red/ET Neomycin Selection Cassette, loxP-flanked, Prokaryotic and Eukaryotic (loxP-PGK-gb2-neo-loxP) | 50ng/ul,20ul | 6077.5 | A001 | Red/ET Neomycin Selection Cassette, Prokaryotic and Eukaryotic (PGK-gb2-neo) | 50ng/ul,20ul | 6077.5 | K001 | Red/ET Quick & Easy BAC Modification Kit (need license to buy) | Kit | 14807 |
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