Nanopsy™是由先進的分子工程納米粒子Magvigen™和Myquvigen™實現的,能夠有效地從蛋白質、細胞和核酸中捕獲和識別分子信息。我們正在將nanopsy™開發成可在成百上千家醫院部署的、自動化和FDA批準的臨床儀器和試劑盒,將致命的癌癥轉變為可控制的疾病。
用鏈霉親和素珠和生物素探針捕獲DNA
Magvigen™、Myquvigen™和Maxvigen™納米顆粒是多功能和可生物降解的磁性和/或熒光磁性納米顆粒,在加利福尼亞大學、伯克利大學、斯坦福大學和NVIGEN經過18年的嚴格研究和開發后達到。
產品名稱 | 應用 | 目錄號 | 規格/價格(1ml, USD) |
MagVigen™ – Plasma DNA Capture Kit | 對血漿中的 cfDNA 的捕獲 | K61003 | Sizes (Plasma) 1,000 ml $3,999.00 |
產品介紹: MagVigen™ Plasma DNA Capture 磁性納米顆粒專為捕獲血液中的cfDNA而設計,為科研, 分子檢測工作者提供了一種簡單、快速、的血液cfDNA提取方法。整個實驗過程中無需離心或者過柱。DNA提取可以線性比例擴大,輸入血漿范圍為100 uL至10mL 。分離出的cfDNA 可以用Agilent Bioanalyzer 直接定量分析。
下游應用: PCR, DNA測序,二代測序技術(NGS), 以及測序文庫構建。
產品特點:
實驗例子:
Figure 1. cfDNA extraction yield and size from 1.5 ml plasma samples with (blue) and without (red) spiking in DNA. Calculated yield of spiking in DNA by subtraction of endogenous cfDNA from total DNA were 80% using picogreen assay and 91% with bioanalyzer quantification.
Figure 2. cfDNA extracted from 100 µl to 5 ml of plasma was quantified by pico-green analysis.
Figure 3. cfDNA extraction yield was quantified by pico-green analysis following DNA isolation with the MagVigen™ Plasma DNA Capture Kit and kit C.
Figure 4. Beta-globin level and GAPDH levels were estimated by PCR analysis.