Visual Protein成立于2005年,致力于通過為研究提供創新產品和服務來加強生物開發。我們的優勢是我們的研發能力。Visual Protein開發的大多數產品比公司開發的產品更有效。我們的專業知識拓寬了蛋白質組學,免疫學和細胞學領域的研究可能性。
VisualProtein BC03-500說明書
Dual-Range™BCA蛋白質分析試劑盒是常用的蛋白質分析方法。它基于二辛可寧酸(BCA),用于比色檢測和定量5-2,000μg/ mL濃度范圍內的總蛋白。與Lowry方法一樣,該分析依賴于蛋白質對Cu 2+離子的還原。由此形成的Cu +通過與二辛可寧酸反應轉化成紫色物質來檢測。Dual-Range™BCA蛋白質分析試劑盒與許多洗滌劑兼容,但與DTT,DTE和2-巰基乙醇等還原劑不相容。
BCA蛋白質測定基于堿性溶液中蛋白質將Cu 2+還原為Cu +,通過二辛可寧酸(BCA)對Cu +進行靈敏且特異性的比色檢測。兩個BCA分子與每個Cu +螯合,使還原的銅從蘋果綠色變為紫色復合物,在562nm處具有強吸收。
貨號 | 產品名稱 | 描述 |
BC03-500 |
Dual-Range™BCA蛋白質分析試劑盒 |
1個套件 500mL試劑A + 12 mL試劑 B + 10mL BSA(2mg / mL)
用于制作2500個微孔板測定
|
圖1.通過使用微孔板程序(37℃,30分鐘)的牛血清白蛋白(BSA)和牛γ球蛋白(BGG)的標準曲線。
圖2.通過使用微孔板程序(37℃,30分鐘),用5%Triton X-100或8%SDS的BSA的標準曲線。
圖3.通過使用
具有增強方案的微孔板程序(60℃持續30分鐘)使用微孔板程序的BSA的標準曲線。
貨號 | 品名 | 規格 |
BC03-500 | Dual-RangeTM BCA protein assay kit | 1 kit |
BR05-500 | Bradford Reagent (5X) | 1 kit |
BR05-500-K | Bradford Protein Assay Kit (5X) | 1 kit |
BP01-1L | BlockPROTM Blocking Buffer | 1 kit |
BF01-1L | BlockPROTM Protein-Free Blocking Buffer | 1 kit |
BF10-100 | BlockPROTM Protein-Free Blocking Buffer (10X) | 1 kit |
BF20-50P | BlockPROTM Protein-Free Blocking Buffer (20X) | 1 kit |
BM01-500 | BlockPRO 1 Min Protein-Free Blocking Buffer | 1 kit |
BM10-100 | BlockPRO 1 Min Protein-Free Blocking Buffer (10X) | 1 kit |
BOX12 | Western Blot Tool Box | 1 kit |
BOX12-03 | Western Blot Tool Box | 1 kit |
EP05-30 | ExtractPROTM Protein Extraction Reagent (Phenol included) | 1 bottle |
FP01-5 | FracPROTM Protein Fractionation Kit | 1 kit |
FP01-50 | FracPROTM Protein Fractionation Kit | 1 kit |
HB01-1L | HybriMoreTM Hybridoma Cloning Factor | 1 bottle |
IF01-4N | ImmunoFastTM Adjuvant | 1 kit |
IF01-20N | ImmunoFastTM Adjuvant | 1 kit |
LF01-10S | LumiFlashTM Prime Chemiluminescent Substrate, HRP (sample size) | 1 kit |
LF01-100 | LumiFlashTM Prime Chemiluminescent Substrate, HRP | 1 kit |
LF01-500 | LumiFlashTM Prime Chemiluminescent Substrate, HRP | 1 kit |
LF08-10S | LumiFlashTM Ultima Chemiluminescent Substrate, HRP (sample size) | 1 kit |
LF08-100 | LumiFlashTM Ultima Chemiluminescent Substrate,HRP | 1 kit |
LF08-500 | LumiFlashTM Ultima Chemiluminescent Substrate,HRP | 1 kit |
LF16-10S | LumiFlashTM Infinity Chemiluminescent Substrate,HRP (sample size) | 1 kit |
LF16-100 | LumiFlashTM Infinity Chemiluminescent Substrate, HRP | 1 kit |
LF16-500 | LumiFlashTM Infinity Chemiluminescent Substrate, HRP | 1 kit |
LH03-10 | LuminolPenTM, HRP System | 1 pen |
LH03-50 | LuminolPenTM, HRP System | 1 pen |
PBS10-1L | PBS Buffer, 10X (pH 7.4) | 1 kit |
PBST10-1L | PBS Buffer, 10X (pH 7.4), with 0.5% Tween 20 | 1 kit |
PP03-2C | PhosPROTM Phosphoprotein Enrichment Kit | 1 kit |
PP03-5E | PhosPROTM phosphoprotein Enrichment Kit | 1 kit |
PP03-6C | PhosPROTM Phosphoprotein Enrichment Kit | 1 kit |
PP05-2C | PhosPROTM Phosphoprotein Purification Kit | 1 kit |
PP05-5E | PhosPROTM Phosphoprotein Purification Kit | 1 kit |
PP05-6C | PhosPROTM Phosphoprotein Purification Kit | 1 kit |
RB10-1L | SDS-PAGE Running Buffer (Tris-Glycine, pH 8.3), 10X | 1 kit |
RB500P | SDS-PAGE Running Buffer (Tris-Glycine), powder pack | 1 kit |
RP05-10 | 5X RIPA Cell Lysis Buffer (sample size) | 1 bottle |
RP05-100 | 5X RIPA Cell Lysis Buffer | 1 bottle |
RP05-500 | 5X RIPA Cell Lysis Buffer | 1 bottle |
SBN06-15 | 6x Laemmli SDS Sample Buffer, non-reducing | 1 kit |
SBR06-15 | 6x Laemmli SDS Sample Buffer, reducing | 1 kit |
SP05-100 | StripPRO 1 Min Stripping Buffer (5X) | 1 kit |
TBS10-1L | TBS Buffer, 10X (pH 7.4) | 1 kit |
TBST10-1L | TBS Buffer, 10X (pH 7.4), with 0.5% Tween 20 | 1 kit |
TBST200P | TBS Buffer, 25X (pH7.4), with 0.5% Tween 20 | 1 kit |
VC01-25S | VisColorTM Pre-Stained Protein Marker | 1 vial |
VC01-250 | VisColorTM Pre-Stained Protein Marker | 1 vial |
VC01-500 | VisColorTM Pre-Stained Protein Marker | 2 vials |
VC03-25S | VisColorTM Full-Range Pre-Stained Protein Marker | 1 vial |
VC03-250 | VisColorTM Full-Range Pre-Stained Protein Marker | 1 vial |
VC03-500 | VisColorTM Full-Range Pre-Stained Protein Marker | 2 vials |
VP01-125 | VisPROTM 5 Minutes Protein Stain Kit (sample size) | 1 kit |
VP01-500 | VisPROTM 5 Minutes Protein Stain Kit | 1 kit |
VP05-125 | 5X VisPROTM 5 Minutes Protein Stain Kit (sample size) | 1 kit |
VP05-500 | 5X VisPROTM 5 Minutes Protein Stain Kit | 1 kit |
WTB10-1L | Western Blot Transfer Buffer (Tris-Glycine, pH 8.3), 10X | 1 kit |
WTB500P | Western Blot Transfer Buffer, powder pack | 1 kit |
BSB01 | Black Staining Box (for VisPRO Protein Stain Kit) | 1 box |
BSB02 | Black Staining Box (for VisPRO Protein Stain Kit) | 1 box |
BSB03 | Black Staining Box (for VisPRO Protein Stain Kit) | 1 box |
BSB04 | Black Staining Box (for VisPRO Protein Stain Kit) | 1box |
上海起發實驗試劑有限公司是實驗試劑一站式采購服務商
1:強大的進口輻射能力,血清、抗體、耗材、大部分限制進口品等。
2:產品種類齊全,經營超過700多品牌,基本涵蓋所有生物實驗試劑耗材。
3:提供加急服務,貨品一般1-2周到貨。
4:富有競爭力的價格優勢,絕大部分價格有優勢。
5:多年積累良好的信譽,大部分客戶提供貨到付款服務??蛻舭ㄇ迦A、北大、交大、復旦、中山等100多所高校,ROCHE,阿斯利康、國藥、fisher等知藥企。
6:我們還是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech;sysy,TriLink BioTechnologies,Inc;worthington-biochem,zyagen等幾十家國外公司授權代理。
7:我們還是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知*批發,歡迎合作。