ESI BIO的使命是成為客戶信賴的合作伙伴,將科學發現轉化為診所。我們通過提供突破性的干細胞技術作為研究級和治療級產品來實現這一目標,從研究到診所的照明,以幫助我們的客戶實現他們治療疾病的目標。
ESI BIO建立在豐富的創新和科學的基礎之上。我們于2000年在新加坡成立了ES Cell International,在那里我們通過制造個可翻譯的臨床級胚胎干細胞系來創建歷史,以便分發給研究界。
ESIBIO現在開展業務Alameda CA. 我們是BioTime,Inc。擁有的以干細胞為基礎的子公司的驕傲家族的成員,我們從中受益于的科學,知識產權和干細胞技術的深層來源。
Product Name | SKU | Price |
HyStem® Hydrogel Trial Kit, 2.5 mL | GS310 | $85.00 |
HyStem® Hydrogel Trial Kit w/ PEGSSDA, 2.5 mL | GS310P | $125.00 |
HyStem® Hydrogel Kit, 7.5 mL | GS311 | $225.00 |
HyStem® Hydrogel Kit w/ PEGSSDA, 7.5 mL | GS311P | $250.00 |
HyStem® Hydrogel Kit, 12.5 mL | GS1004 | $275.00 |
HyStem ®水凝膠-的空白石板矩陣。*可定制和無xeno。組分包括巰基修飾的透明質酸(Gycosil ®),巰基反應性交聯劑PEGDA(Extralink ®),并脫氣,去離子水(DG水)。
HyStem ®水凝膠是無異物的,可*定制。推薦用于需要特定附著因子優化以及非貼壁細胞培養,細胞應用的應用。細胞外基質蛋白可以混合到水凝膠中并在凝膠化之前非共價地摻入?;蛘?,具有N-末端半胱氨酸的連接肽也可以與基質共價連接。HyStem水凝膠試劑盒是培養干細胞的宜選擇,其天然環境富含透明質酸(HA)。HyStem水凝膠可以通過將細胞外基質(ECM)蛋白或細胞附著肽添加到水凝膠中來定制,以提供附著位點和/或分化信號。它們也可以通過改變水凝膠的剛性來改變,以匹配天然細胞環境。
特征
· 尺寸(水凝膠頂部的3-D封裝或2-D電鍍)
· 靈活的培養形式(384至6孔板,組織培養插入物和T型瓶)
· 摻入可變量和類型的ECM蛋白
· 凝膠特性包括膠凝時間和水凝膠硬度
無動物成分的系統
,因為它的兩個組件是巰基修飾的透明質酸(Glycosil HyStem水凝膠是無異物®)和巰基反應性交聯劑(聚乙二醇二丙烯酸酯,Extralink ® -Lite)。用于生產HyStem的透明質酸是通過使用枯草芽孢桿菌(Novozymes)的專有細菌發酵方法制備的。它100%不含動物源性原料,并且在其生產中不使用動物源性成分。Extralink(聚乙二醇二丙烯酸酯)通過在聚乙二醇(PEG)聚合物的兩端添加丙烯酸酯基團來制備。PEG衍生自石油和無機來源,不含動物源材料。
凝膠化
重構的HyStem-C組分在15至37℃下保持液態數小時。當交聯劑,Extralink在水凝膠形成® -Lite(PEGDA)加入到Glycosil的混合物®(巰基修飾的透明質酸。所有三種成分混合后,會發生在大約二十分鐘膠凝。否步驟取決于低溫或低pH值。用磷酸鹽緩沖鹽水(PBS)或細胞培養基稀釋組分可以增加凝膠時間
.3D細胞回收矩陣
對于細胞回收至關重要的應用,替代交聯劑PEGSSDA可用于所有HyStem水凝膠試劑盒。這種交聯劑具有Extralink提供的相同優點,還具有易于還原的內部鍵的額外優點。這允許從水凝膠中快速,容易地回收單細胞或簇,用于RNA分析或流式細胞術等應用,而不是可影響細胞活力的慢酶法。鼓勵研究人員與我們聯系,以確定特定細胞類型或培養系統與PEGSSDA的相容性。
儲存和穩定性 | 將Glycosil在-20°C的原始樣品瓶中保存長達一年。 |
技術文件 | GS310技術數據表 產品支持 HyStem Hydrogels常見問題解答 小冊子 HyStem ®水凝膠 |
組件 | Extralink ® |
參考 | 舒,XZ,等。(2004)用于組織工程的原位可交聯透明質酸水凝膠。Biomaterials 25:1339-1348。PMID:14643608. |
ESI BIO產品目錄:
貨號 | 品名 | 規格 | 品牌 |
GS310 | HyStem® Hydrogel Kit | 2.5 mL | ESIBIO |
GS311 | HyStem® Hydrogel Kit | 7.5 mL | ESIBIO |
GS1004 | HyStem® Hydrogel Kit | 12.5 mL | ESIBIO |
GS310P | HyStem® Hydrogel Kit w/ PEGSSDA | 2.5 mL | ESIBIO |
GS311P | HyStem® Hydrogel Kit w/ PEGSSDA | 7.5 mL | ESIBIO |
GS312 | HyStem®-C Hydrogel Kit | 2.5 mL | ESIBIO |
GS313 | HyStem®-C Hydrogel Kit | 7.5 mL | ESIBIO |
GS1005 | HyStem®-C Hydrogel Kit | 12.5 mL | ESIBIO |
GS312P | HyStem®-C Hydrogel Kit w/ PEGSSDA | 2.5 mL | ESIBIO |
GS313P | HyStem®-C Hydrogel Kit w/ PEGSSDA | 7.5 mL | ESIBIO |
GS314 | HyStem®-HP Hydrogel Kit | 2.5 mL | ESIBIO |
GS315 | HyStem®-HP Hydrogel Kit | 7.5 mL | ESIBIO |
GS1006 | HyStem®-HP Hydrogel Kit | 12.5 mL | ESIBIO |
GS314P | HyStem®-HP Hydrogel Kit w/ PEGSSDA | 2.5 mL | ESIBIO |
GS315P | HyStem®-HP Hydrogel Kit w/ PEGSSDA | 7.5 mL | ESIBIO |
GS1007 | HyStem® Hydrogel UV QuickSet Kit | 2.5 mL | ESIBIO |
GS1008 | HyStem® Hydrogel UV QuickSet Kit | 7.5 mL | ESIBIO |
GS450 | PEGgel Kit | 1 mL | ESIBIO |
GS240 | DG Water | 10 mL | ESIBIO |
GS241 | DG Water | 20 mL | ESIBIO |
GS3007 | Extralink® Vial | 0.5 mL | ESIBIO |
GS3006 | Extralink® Vial | 2.5 mL | ESIBIO |
GS3009 | Extralink® Lite Vial | 0.5 mL | ESIBIO |
GS3008 | Extralink® Lite Vial | 2.5 mL | ESIBIO |
5050 | Fibronectin | 1 mg | ESIBIO |
GS231 | Gelin-S® Thiol-modified Gelatin | 1 mL | ESIBIO |
GS230 | Gelin-S® Thiol-modified Gelatin | 5 mL | ESIBIO |
GS222 | Glycosil® Hyaluronic Acid | 1 mL | ESIBIO |
GS220 | Glycosil® Hyaluronic Acid | 5 mL | ESIBIO |
GS217 | Heprasil® Hyaluronic Acid | 1 mL | ESIBIO |
GS215 | Heprasil® Hyaluronic Acid | 5 mL | ESIBIO |
5010-D | Nutragen® Bovine Collagen | 50 mL | ESIBIO |
GS711 | PEGDA | 1 mL | ESIBIO |
GS700 | PEGDA | 1 g | ESIBIO |
GS705 | PEGDA | 5 g | ESIBIO |
GS755 | PEGSSDA | 0.5 mL | ESIBIO |
5020 | PEPTITE-2000® RGD Peptide | 5 mg | ESIBIO |
5005-B | PureCol® Collagen | 100 mL | ESIBIO |
5007-A | VitroCol® Collagen | 20 mL | ESIBIO |
5051 | Vitronectin | 0.1 mg | ESIBIO |
EM2203 | ExoSense™ CD63 Exosome ELISA Kit | 96 assays | ESIBIO |
EM2202 | VascuNet™ CoCulture Assay Kit | 1 Kit | ESIBIO |
ST11006 | BioLiteTM SSEA-1 (DyLight 488) anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11008 | BioLiteTM TRA-1-81 (DyLight 488) anti-Human Antibody | 100 µL | ESIBIO |
ST11023 | Nestin anti-Human Antibody | 100 µL | ESIBIO |
ST11003 | Oct4 anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11001 | Sox2 anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11013 | SSEA-1 anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11014 | SSEA-3 anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11015 | SSEA-4 anti-Human Antibody | 100 µL | ESIBIO |
ST11018 | TRA-1-60 (PE) anti-Human Antibody | 100 µL | ESIBIO |
ST11016 | TRA-1-60 anti-Human Antibody | 100 µL | ESIBIO |
ST11017 | TRA-1-81 anti-Human Antibody | 100 µL | ESIBIO |
ST12007 | LIF, Mouse Recombinant | 10 µg | ESIBIO |
ST12008 | LIF, Mouse Recombinant | 100 µg | ESIBIO |
ES-84 | PureStem® 4D20.8, NCr-fac Progenitor | ea | ESIBIO |
ES-98 | PureStem® E15, Meso-prx/latp Progenitor | ea | ESIBIO |
ES-283 | PureStem® 7PEND24, NCr-fac Progenitor | ea | ESIBIO |
ES-278 | PureStem® 7SMOO32, NCr-fac Progenitor | ea | ESIBIO |
ES-250 | PureStem® SK11, NCr-fac Progenitor | ea | ESIBIO |
ES-268 | PureStem® MEL2, NCr-fac Progenitor | ea | ESIBIO |
ES-256 | PureStem® SM30, NCr-fac Progenitor | ea | ESIBIO |
ES-335 | PureStem® ES-335 Meso-latp Progenitor | ea | ESIBIO |
ES-100 | PureStem® E72 BETATROPHIN+ Progenitor | ea | ESIBIO |
ES-1001 | PureStem® ES-1001 GDF11+ Progenitor | ea | ESIBIO |
ES-184 | PureStem® EN7 Progenitor | ea | ESIBIO |
ES-101 | PureStem® ES-101 Progenitor | ea | ESIBIO |
ES-139 | PureStem® ES-139 Progenitor | ea | ESIBIO |
ES-154 | PureStem® ES-154 Progenitor | ea | ESIBIO |
ES-198 | PureStem® ES-198 Progenitor | ea | ESIBIO |
ES-199 | PureStem® ES-199 Progenitor | ea | ESIBIO |
ES-209 | PureStem® ES-209, Meso-prx/latp Progenitor | ea | ESIBIO |
ES-210 | PureStem® ES-210, Ecto-ntu Progenitor | ea | ESIBIO |
ES-236 | PureStem® ES-236 Progenitor | ea | ESIBIO |
ES-170 | PureStem® E44 Progenitor | ea | ESIBIO |
ES-196 | PureStem® W10 Progenitor | ea | ESIBIO |
ES-194 | PureStem® Z11, Meso Progenitor | ea | ESIBIO |
EM-1001 | PureStem® Progenitor Growth Media, EPM k01 | 500 mL | ESIBIO |
EM-1002 | PureStem® Progenitor Growth Media, EPM k02 | 500 mL | ESIBIO |
EM-1003 | PureStem® Progenitor Growth Media, EPM k03 | 500 mL | ESIBIO |
EM-1004 | PureStem® Progenitor Growth Media, EPM k04 | 500 mL | ESIBIO |
EM-1005 | PureStem® Progenitor Growth Media, EPM k05 | 500 mL | ESIBIO |
EM-1006 | PureStem® Progenitor Growth Media, EPM k06 | 500 mL | ESIBIO |
EM-1007 | PureStem® Progenitor Growth Media, EPM k07 | 500 mL | ESIBIO |
EM-1008 | PureStem® Progenitor Growth Media, EPM k08 | 500 mL | ESIBIO |
EM-2002 | HyStem®-4D Chondrogenesis Differentiation Kit | 1 Kit | ESIBIO |
EM-2007 | HyStem®-4D Differentiation Kit | 1 Kit | ESIBIO |
EM-2001 | PureStem® Chondrogenesis Differentiation Kit | 1 Kit | ESIBIO |
EM-2006 | PureStem® Choroid Plexus Differentiation Kit | 1 Kit | ESIBIO |
EM-2003 | PureStem® Osteogenesis Differentiation Kit 01 | 1 Kit | ESIBIO |
EM-2004 | PureStem® Osteogenesis Differentiation Kit 02 | 1 Kit | ESIBIO |
ST10035 | LY411575 | 5 mg | ESIBIO |
ST10008 | PD0325901 | 2 mg | ESIBIO |
ST10009 | PD0325901 | 10 mg | ESIBIO |
ST10010 | PD0325901 | 415 µL, 10 mM | ESIBIO |
ST10034 | PD173074 | 2 mg | ESIBIO |
ST10021 | RepSox | 5 mg | ESIBIO |
ST10027 | RG108 | 5 mg | ESIBIO |
ST10024 | SB203580 | 2 mg | ESIBIO |
ST10012 | SB431542 | 5 mg | ESIBIO |
ST10013 | SB431542 | 10 mg | ESIBIO |
ST10014 | SB431542 | 1.3 mL, 10 mM | ESIBIO |
ST10025 | SP600125 | 5 mg | ESIBIO |
ST10015 | Thiazovivin | 2 mg | ESIBIO |
ST10017 | XAV939 | 2 mg | ESIBIO |
ST10018 | Y27632 | 2 mg | ESIBIO |
ST10019 | Y27632 | 10 mg | ESIBIO |
ST10020 | Y27632 | 625 µL, 10 mM | ESIBIO |
上海起發實驗試劑有限公司是實驗試劑一站式采購服務商
1:強大的進口輻射能力,血清、抗體、耗材、大部分限制進口品等。
2:產品種類齊全,經營超過700多品牌,基本涵蓋所有生物實驗試劑耗材。
3:提供加急服務,貨品一般1-2周到貨。
4:富有競爭力的價格優勢,絕大部分價格有優勢。
5:多年積累良好的信譽,大部分客戶提供貨到付款服務??蛻舭ㄇ迦A、北大、交大、復旦、中山等100多所高校,ROCHE,阿斯利康、國藥、fisher等知藥企。
6:我們還是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech;sysy,TriLink BioTechnologies,Inc;worthington-biochem,zyagen等幾十家國外公司授權代理。
7:我們還是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知*批發,歡迎合作。