ESI BIO的使命是成為客戶信賴的合作伙伴,將科學發現轉化為診所。我們通過提供突破性的干細胞技術作為研究級和治療級產品來實現這一目標,從研究到診所的照明,以幫助我們的客戶實現他們治療疾病的目標。
ESI BIO建立在豐富的創新和科學的基礎之上。我們于2000年在新加坡成立了ES Cell International,在那里我們通過制造個可翻譯的臨床級胚胎干細胞系來創建歷史,以便分發給研究界。
ESI BIO現在開展業務Alameda CA. 我們是BioTime,Inc。擁有的以干細胞為基礎的子公司的驕傲家族的成員,我們從中受益于的科學,知識產權和干細胞技術的深層來源。
Product Name | SKU | Price |
HyStem®-C Hydrogel Trial Kit, 2.5 mL | GS312 | $85.00 |
HyStem®-C Hydrogel Trial Kit w/ PEGSSDA, 2.5 mL | GS312P | $125.00 |
HyStem®-C Hydrogel Kit, 7.5 mL | GS313 | $195.00 |
HyStem®-C Hydrogel Kit w/ PEGSSDA, 7.5 mL | GS313P | $225.00 |
HyStem®-C Hydrogel Kit, 12.5 mL | GS1005 | $245.00 |
HyStem ® -C水凝膠為用于細胞培養基質優化提供一個很好的起點。HyStem-C*化學成分確定的和基于三個生物相容性組件:巰基修飾的透明質酸(Glycosil),巰基反應性交聯劑,PEGDA(Extralink),以及巰基修飾的膠原變性(格林-S ®)。Gelin-S為各種原代細胞和細胞系提供了基本的細胞附著位點,因此被推薦作為貼壁細胞類型和細胞培養優化的理想底物。在某些情況下,可以通過添加ECM蛋白來進一步增強HyStem-C水凝膠以匹配天然信號。
特征
· 水凝膠適用于培養原代細胞,干細胞和細胞系。
· 細胞可以以任何形式包封或生長在水凝膠表面上,包括培養瓶,6-至384孔板或組織培養插入物。
· 通過操縱組分濃度和混合比,使用者可以容易地定制水凝膠以具有所需的硬度和凝膠化時間。
· 可定制的凝膠特性,包括凝膠時間和水凝膠硬度。
凝膠化
重組的HyStem-C組分在15至37℃保持液態。當交聯劑,Extralink在水凝膠形成®(PEGDA)加入到Glycosil的混合物®(巰基修飾的透明質酸)和格林-S ®(巰基修飾的明膠)。在所有三種組分混合后約20分鐘發生凝膠化。沒有步驟取決于低溫或低pH。用磷酸鹽緩沖鹽水(PBS)或細胞培養基稀釋組分可以增加凝膠化時間。
3D細胞恢復矩陣
對于細胞回收至關重要的應用,替代交聯劑PEGSSDA可用于所有HyStem,HyStem-C和HyStem-HP試劑盒。這種交聯劑具有Extralink提供的相同優點,還具有易于還原的內部鍵的額外優點。這允許從水凝膠中快速,容易地回收單細胞或簇,用于RNA分析或流式細胞術等應用,而不是可影響細胞活力的慢酶法。鼓勵研究人員與我們聯系,以確定特定細胞類型或培養系統與PEGSSDA的相容性。
儲存和穩定性 | 將Glycosil和Gelin-S在-20°C的原始樣品瓶中保存長達一年。 |
技術文件 | GS312技術數據表 產品支持 HyStem Hydrogels常見問題解答 小冊子 HyStem ®水凝膠 |
組件 | Extralink ® |
參考 | 新!Gaetani,R.,et al。(2015)心臟祖細胞在3D打印的明膠/透明質酸貼劑中的心外膜應用保留了心肌梗塞后的心臟功能。Biomaterials 61:339-348。PMID:17335875。Prestwich |
ESI BIO產品目錄:
貨號 | 品名 | 規格 | 品牌 |
GS310 | HyStem® Hydrogel Kit | 2.5 mL | ESIBIO |
GS311 | HyStem® Hydrogel Kit | 7.5 mL | ESIBIO |
GS1004 | HyStem® Hydrogel Kit | 12.5 mL | ESIBIO |
GS310P | HyStem® Hydrogel Kit w/ PEGSSDA | 2.5 mL | ESIBIO |
GS311P | HyStem® Hydrogel Kit w/ PEGSSDA | 7.5 mL | ESIBIO |
GS312 | HyStem®-C Hydrogel Kit | 2.5 mL | ESIBIO |
GS313 | HyStem®-C Hydrogel Kit | 7.5 mL | ESIBIO |
GS1005 | HyStem®-C Hydrogel Kit | 12.5 mL | ESIBIO |
GS312P | HyStem®-C Hydrogel Kit w/ PEGSSDA | 2.5 mL | ESIBIO |
GS313P | HyStem®-C Hydrogel Kit w/ PEGSSDA | 7.5 mL | ESIBIO |
GS314 | HyStem®-HP Hydrogel Kit | 2.5 mL | ESIBIO |
GS315 | HyStem®-HP Hydrogel Kit | 7.5 mL | ESIBIO |
GS1006 | HyStem®-HP Hydrogel Kit | 12.5 mL | ESIBIO |
GS314P | HyStem®-HP Hydrogel Kit w/ PEGSSDA | 2.5 mL | ESIBIO |
GS315P | HyStem®-HP Hydrogel Kit w/ PEGSSDA | 7.5 mL | ESIBIO |
GS1007 | HyStem® Hydrogel UV QuickSet Kit | 2.5 mL | ESIBIO |
GS1008 | HyStem® Hydrogel UV QuickSet Kit | 7.5 mL | ESIBIO |
GS450 | PEGgel Kit | 1 mL | ESIBIO |
GS240 | DG Water | 10 mL | ESIBIO |
GS241 | DG Water | 20 mL | ESIBIO |
GS3007 | Extralink® Vial | 0.5 mL | ESIBIO |
GS3006 | Extralink® Vial | 2.5 mL | ESIBIO |
GS3009 | Extralink® Lite Vial | 0.5 mL | ESIBIO |
GS3008 | Extralink® Lite Vial | 2.5 mL | ESIBIO |
5050 | Fibronectin | 1 mg | ESIBIO |
GS231 | Gelin-S® Thiol-modified Gelatin | 1 mL | ESIBIO |
GS230 | Gelin-S® Thiol-modified Gelatin | 5 mL | ESIBIO |
GS222 | Glycosil® Hyaluronic Acid | 1 mL | ESIBIO |
GS220 | Glycosil® Hyaluronic Acid | 5 mL | ESIBIO |
GS217 | Heprasil® Hyaluronic Acid | 1 mL | ESIBIO |
GS215 | Heprasil® Hyaluronic Acid | 5 mL | ESIBIO |
5010-D | Nutragen® Bovine Collagen | 50 mL | ESIBIO |
GS711 | PEGDA | 1 mL | ESIBIO |
GS700 | PEGDA | 1 g | ESIBIO |
GS705 | PEGDA | 5 g | ESIBIO |
GS755 | PEGSSDA | 0.5 mL | ESIBIO |
5020 | PEPTITE-2000® RGD Peptide | 5 mg | ESIBIO |
5005-B | PureCol® Collagen | 100 mL | ESIBIO |
5007-A | VitroCol® Collagen | 20 mL | ESIBIO |
5051 | Vitronectin | 0.1 mg | ESIBIO |
EM2203 | ExoSense™ CD63 Exosome ELISA Kit | 96 assays | ESIBIO |
EM2202 | VascuNet™ CoCulture Assay Kit | 1 Kit | ESIBIO |
ST11006 | BioLiteTM SSEA-1 (DyLight 488) anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11008 | BioLiteTM TRA-1-81 (DyLight 488) anti-Human Antibody | 100 µL | ESIBIO |
ST11023 | Nestin anti-Human Antibody | 100 µL | ESIBIO |
ST11003 | Oct4 anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11001 | Sox2 anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11013 | SSEA-1 anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11014 | SSEA-3 anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11015 | SSEA-4 anti-Human Antibody | 100 µL | ESIBIO |
ST11018 | TRA-1-60 (PE) anti-Human Antibody | 100 µL | ESIBIO |
ST11016 | TRA-1-60 anti-Human Antibody | 100 µL | ESIBIO |
ST11017 | TRA-1-81 anti-Human Antibody | 100 µL | ESIBIO |
ST12007 | LIF, Mouse Recombinant | 10 µg | ESIBIO |
ST12008 | LIF, Mouse Recombinant | 100 µg | ESIBIO |
ES-84 | PureStem® 4D20.8, NCr-fac Progenitor | ea | ESIBIO |
ES-98 | PureStem® E15, Meso-prx/latp Progenitor | ea | ESIBIO |
ES-283 | PureStem® 7PEND24, NCr-fac Progenitor | ea | ESIBIO |
ES-278 | PureStem® 7SMOO32, NCr-fac Progenitor | ea | ESIBIO |
ES-250 | PureStem® SK11, NCr-fac Progenitor | ea | ESIBIO |
ES-268 | PureStem® MEL2, NCr-fac Progenitor | ea | ESIBIO |
ES-256 | PureStem® SM30, NCr-fac Progenitor | ea | ESIBIO |
ES-335 | PureStem® ES-335 Meso-latp Progenitor | ea | ESIBIO |
ES-100 | PureStem® E72 BETATROPHIN+ Progenitor | ea | ESIBIO |
ES-1001 | PureStem® ES-1001 GDF11+ Progenitor | ea | ESIBIO |
ES-184 | PureStem® EN7 Progenitor | ea | ESIBIO |
ES-101 | PureStem® ES-101 Progenitor | ea | ESIBIO |
ES-139 | PureStem® ES-139 Progenitor | ea | ESIBIO |
ES-154 | PureStem® ES-154 Progenitor | ea | ESIBIO |
ES-198 | PureStem® ES-198 Progenitor | ea | ESIBIO |
ES-199 | PureStem® ES-199 Progenitor | ea | ESIBIO |
ES-209 | PureStem® ES-209, Meso-prx/latp Progenitor | ea | ESIBIO |
ES-210 | PureStem® ES-210, Ecto-ntu Progenitor | ea | ESIBIO |
ES-236 | PureStem® ES-236 Progenitor | ea | ESIBIO |
ES-170 | PureStem® E44 Progenitor | ea | ESIBIO |
ES-196 | PureStem® W10 Progenitor | ea | ESIBIO |
ES-194 | PureStem® Z11, Meso Progenitor | ea | ESIBIO |
EM-1001 | PureStem® Progenitor Growth Media, EPM k01 | 500 mL | ESIBIO |
EM-1002 | PureStem® Progenitor Growth Media, EPM k02 | 500 mL | ESIBIO |
EM-1003 | PureStem® Progenitor Growth Media, EPM k03 | 500 mL | ESIBIO |
EM-1004 | PureStem® Progenitor Growth Media, EPM k04 | 500 mL | ESIBIO |
EM-1005 | PureStem® Progenitor Growth Media, EPM k05 | 500 mL | ESIBIO |
EM-1006 | PureStem® Progenitor Growth Media, EPM k06 | 500 mL | ESIBIO |
EM-1007 | PureStem® Progenitor Growth Media, EPM k07 | 500 mL | ESIBIO |
EM-1008 | PureStem® Progenitor Growth Media, EPM k08 | 500 mL | ESIBIO |
EM-2002 | HyStem®-4D Chondrogenesis Differentiation Kit | 1 Kit | ESIBIO |
EM-2007 | HyStem®-4D Differentiation Kit | 1 Kit | ESIBIO |
EM-2001 | PureStem® Chondrogenesis Differentiation Kit | 1 Kit | ESIBIO |
EM-2006 | PureStem® Choroid Plexus Differentiation Kit | 1 Kit | ESIBIO |
EM-2003 | PureStem® Osteogenesis Differentiation Kit 01 | 1 Kit | ESIBIO |
EM-2004 | PureStem® Osteogenesis Differentiation Kit 02 | 1 Kit | ESIBIO |
ST10035 | LY411575 | 5 mg | ESIBIO |
ST10008 | PD0325901 | 2 mg | ESIBIO |
ST10009 | PD0325901 | 10 mg | ESIBIO |
ST10010 | PD0325901 | 415 µL, 10 mM | ESIBIO |
ST10034 | PD173074 | 2 mg | ESIBIO |
ST10021 | RepSox | 5 mg | ESIBIO |
ST10027 | RG108 | 5 mg | ESIBIO |
ST10024 | SB203580 | 2 mg | ESIBIO |
ST10012 | SB431542 | 5 mg | ESIBIO |
ST10013 | SB431542 | 10 mg | ESIBIO |
ST10014 | SB431542 | 1.3 mL, 10 mM | ESIBIO |
ST10025 | SP600125 | 5 mg | ESIBIO |
ST10015 | Thiazovivin | 2 mg | ESIBIO |
ST10017 | XAV939 | 2 mg | ESIBIO |
ST10018 | Y27632 | 2 mg | ESIBIO |
ST10019 | Y27632 | 10 mg | ESIBIO |
ST10020 | Y27632 | 625 µL, 10 mM | ESIBIO |
上海起發實驗試劑有限公司是實驗試劑一站式采購服務商
1:強大的進口輻射能力,血清、抗體、耗材、大部分限制進口品等。
2:產品種類齊全,經營超過700多品牌,基本涵蓋所有生物實驗試劑耗材。
3:提供加急服務,貨品一般1-2周到貨。
4:富有競爭力的價格優勢,絕大部分價格有優勢。
5:多年積累良好的信譽,大部分客戶提供貨到付款服務。客戶包括清華、北大、交大、復旦、中山等100多所高校,ROCHE,阿斯利康、國藥、fisher等知藥企。
6:我們還是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech;sysy,TriLink BioTechnologies,Inc;worthington-biochem,zyagen等幾十家國外公司授權代理。
7:我們還是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知*批發,歡迎合作。