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Cyandye Lot 10002說明書

 更新時間:2018-06-21 點擊量:1129

上海起發為Cyandye特約經銷商,Cyandye是一家以合理的價格向處理分子生物學的科學家提供化學服務的公司。Cyandye的專長是生物技術和生命科學的熒光染料。

 

Cyandye  Lot 10002說明書:

 

 

貨號

品名

規格

品牌

 Lot 10002

Azidobutyrate NHS Ester

50 mg

cyandye

 Lot 10002

Azidobutyrate NHS Ester

100 mg

cyandye

 Lot 10002

Azidobutyrate NHS Ester

250 mg

cyandye

 Lot 10002

Azidobutyrate NHS Ester

50 mg

cyandye

 

 

分子式:C 8 H 10 N 4 O 4分子量
:226.19 
同義詞:

  • 1 - [(4- azidobutanoyl)氧基]吡咯烷-2,5-二酮
  • 琥珀酰亞胺4-疊氮丁酸酯
  • NHS-C3疊氮
  • 疊氮丁酸氧基琥珀酰亞胺酯>

 

對于標記0.2微摩爾的氨基修飾的寡核苷酸,在60μL的ACN中溶解2.3mg的疊氮丁酸NHS酯(DMSO或DMF也是可接受的)。將氨基修飾的寡核苷酸溶解在500μL的0.1M碳酸鹽/碳酸氫鹽緩沖液(pH 9)中。加入12μL的Azidobutyrate NHS酯溶液到溶解的寡核苷酸中,并在室溫下反應2小時并脫鹽。

儲存:干燥,-10至-30°C 
稀釋劑:無水ACN,DMSO或DMF溶液
穩定性:2-3天

質量控制: NMR:95%TLC:純

AZIDOBUTYRATE使用

在這種情況下,在任何合成器上像往常一樣合成寡核苷酸。氨基必須在寡核苷酸的3'或5'端引入。在裂解和脫保護之后,疊氮基可以作為胺的修飾被引入。例如,碳酸鈉/碳酸氫鈉緩沖液(pH8.75)中的寡核苷酸在室溫下與琥珀酰亞胺基-4-疊氮基丁酸酯在DMSO中孵育。然后使用HPLC純化終的寡核苷酸或用乙醇或丁醇沉淀。

參考文獻:
J. Am?;瘜W。
使用Click Chemistry
Langmuir,2010,26(11),pp 8666-8672 DOI:10.1021 / ja070273v Template-Directed Oligonucleotide Strand Ligation,Covalent Intramolecular DNA Circularization and Catenation Using Click Chemistry Langmuir,2010,26(11),pp 8666-8672 DOI: 10.1021 / la904822f 
將脂質囊泡共價連接到液體支持的雙層允許觀察DNA介導的囊泡相互作用

 

 

 

Cyandye 產品列表:

 

貨號

品名

規格

品牌

 Lot 10002

Azidobutyrate NHS Ester

50 mg

cyandye

 Lot 10002

Azidobutyrate NHS Ester

100 mg

cyandye

 Lot 10002

Azidobutyrate NHS Ester

250 mg

cyandye

 Lot 10007

Cy3 carboxylic acid

5 mg

cyandye

 Lot 10007

Cy3 carboxylic acid

100 mg

cyandye

Lot 10006

Cy5 carboxylic acid

5 mg

cyandye

Lot 10012

Cy5.5 carboxylic acid

5 mg

cyandye

Lot 10012

Cy5.5 carboxylic acid

25 mg

cyandye

Lot 10012

Cy5.5 carboxylic acid

50 mg

cyandye

Lot 10012

Cy5.5 carboxylic acid

100 mg

cyandye

 Lot 10013

diSulfo - Cy3 alkyne

1 mg

cyandye

 Lot 10013

diSulfo - Cy3 alkyne

5 mg

cyandye

 Lot 10013

diSulfo - Cy3 alkyne

10 mg

cyandye

 Lot 10013

diSulfo - Cy3 alkyne

25 mg

cyandye

Lot 10022

diSulfo - Cy3 azide

1 mg

cyandye

Lot 10022

diSulfo - Cy3 azide

5 mg

cyandye

Lot 10022

diSulfo - Cy3 azide

10 mg

cyandye

Lot 10022

diSulfo - Cy3 azide

25 mg

cyandye

Lot a0015

diSulfo - Cy3 carboxylic acid

5 mg

cyandye

Lot a0015

diSulfo - Cy3 carboxylic acid

25 mg

cyandye

Lot a0015

diSulfo - Cy3 carboxylic acid

50 mg

cyandye

Lot a0015

diSulfo - Cy3 carboxylic acid

100 mg

cyandye

Lot 10017

diSulfo - Cy3 NHS Ester

5 mg

cyandye

Lot 10017

diSulfo - Cy3 NHS Ester

25 mg

cyandye

Lot 10017

diSulfo - Cy3 NHS Ester

50 mg

cyandye

Lot 10017

diSulfo - Cy3 NHS Ester

100 mg

cyandye

Lot 10016

diSulfo - Cy5 alkyne

1 mg

cyandye

Lot 10016

diSulfo - Cy5 alkyne

5 mg

cyandye

Lot 10016

diSulfo - Cy5 alkyne

10 mg

cyandye

Lot 10016

diSulfo - Cy5 alkyne

25 mg

cyandye

 Lot 10023

diSulfo - Cy5 azide

1 mg

cyandye

 Lot 10023

diSulfo - Cy5 azide

5 mg

cyandye

 Lot 10010

diSulfo - Cy5 carboxylic acid

5 mg

cyandye

 Lot 10010

diSulfo - Cy5 carboxylic acid

100 mg

cyandye

Lot 10009

diSulfo - Cy5 Hydrazide

5 mg

cyandye

Lot 10009

diSulfo - Cy5 Hydrazide

25 mg

cyandye

Lot 10009

diSulfo - Cy5 Hydrazide

50 mg

cyandye

Lot 10009

diSulfo - Cy5 Hydrazide

100 mg

cyandye

Lot 10014

diSulfo - Cy5 NHS Ester

5 mg

cyandye

Lot 10014

diSulfo - Cy5 NHS Ester

25 mg

cyandye

Lot 10014

diSulfo - Cy5 NHS Ester

50 mg

cyandye

Lot 10014

diSulfo - Cy5 NHS Ester

100 mg

cyandye

Lot 10027

diSulfo - Cy7 carboxylic acid

5 mg

cyandye

Lot a0021

diSulfo - Cy7 NHS Ester

5 mg

cyandye

Lot a0021

diSulfo - Cy7 NHS Ester

25 mg

cyandye

Lot a0021

diSulfo - Cy7 NHS Ester

50 mg

cyandye

Lot a0021

diSulfo - Cy7 NHS Ester

100 mg

cyandye

Lot 10004

monoSulfo - Cy3 alkyne

1 mg

cyandye

Lot 10004

monoSulfo - Cy3 alkyne

5 mg

cyandye

Lot 10004

monoSulfo - Cy3 alkyne

10 mg

cyandye

Lot 10004

monoSulfo - Cy3 alkyne

100 mg

cyandye

Lot 10011

monoSulfo - Cy3 azide

1 mg

cyandye

Lot 10011

monoSulfo - Cy3 azide

5 mg

cyandye

Lot 10011

monoSulfo - Cy3 azide

10 mg

cyandye

Lot 10011

monoSulfo - Cy3 azide

100 mg

cyandye

Lot 10025

monoSulfo - Cy3 carboxylic acid

5 mg

cyandye

Lot 10025

monoSulfo - Cy3 carboxylic acid

25 mg

cyandye

Lot 10025

monoSulfo - Cy3 carboxylic acid

50 mg

cyandye

Lot 10025

monoSulfo - Cy3 carboxylic acid

100 mg

cyandye

Lot 10008

monoSulfo - Cy3 NHS Ester

5 mg

cyandye

Lot 10008

monoSulfo - Cy3 NHS Ester

100 mg

cyandye

Lot 10020

monoSulfo - Cy5 azide

1 mg

cyandye

Lot 10020

monoSulfo - Cy5 azide

5 mg

cyandye

Lot 10020

monoSulfo - Cy5 azide

10 mg

cyandye

Lot 10020

monoSulfo - Cy5 azide

100 mg

cyandye

 Lot 10026

monoSulfo - Cy5 carboxylic acid

5 mg

cyandye

 Lot 10026

monoSulfo - Cy5 carboxylic acid

25 mg

cyandye

 Lot 10018

monoSulfo - Cy5 NHS Ester

5 mg

cyandye

 Lot 10018

monoSulfo - Cy5 NHS Ester

25 mg

cyandye

Lot a0024

3-azidopropylamine

100 mg

cyandye

 Lot d2501

2'-Deoxy-5'-acetyl-5-acetoxymethyluridine

250 mg

cyandye

 Lot a0001

2'-Deoxy-5-hydroxymethyluridine

250 mg

cyandye

 Lot a0001

2'-Deoxy-5-hydroxymethyluridine

500 mg

cyandye

Lot a0029

4-hydrazinobenzene-1,3-disulfonic acid

500 mg

cyandye

Lot a0028

5-(hydroxymethyl)-1-[(6aR,8R,9aS)-2,2,4,4-tetraisopropyltetrahydro-6H-furo[3,2-f][1,3,5,2,4]trioxadisilocin-8-yl]pyrimidine-2,4(1H,3H)-dione

50 mg

cyandye

Lot a0019

2,3,6,7-tetrahydro-1H,5H-pyrido[3,2,1-ij]quinolin-9-ol

500 mg

cyandye

Lot a0003

2,3,4,6-tetra-O-benzoyl-1-O-(2,2,2-trichloroethanimidoyl)-α-D-glucopyranose

50 mg

cyandye

Lot a0003

2,3,4,6-tetra-O-benzoyl-1-O-(2,2,2-trichloroethanimidoyl)-α-D-glucopyranose

100 mg

cyandye

Lot a0003

2,3,4,6-tetra-O-benzoyl-1-O-(2,2,2-trichloroethanimidoyl)-α-D-glucopyranose

250 mg

cyandye

 

 

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