上海起發為Cyandye特約經銷商,Cyandye是一家以合理的價格向處理分子生物學的科學家提供化學服務的公司。Cyandye的專長是生物技術和生命科學的熒光染料。
貨號 | 品名 | 規格 | 品牌 |
Lot 10002 | Azidobutyrate NHS Ester | 50 mg | cyandye |
Lot 10002 | Azidobutyrate NHS Ester | 100 mg | cyandye |
Lot 10002 | Azidobutyrate NHS Ester | 250 mg | cyandye |
Lot 10002 | Azidobutyrate NHS Ester | 50 mg | cyandye |
分子式:C 8 H 10 N 4 O 4分子量
:226.19
同義詞:
對于標記0.2微摩爾的氨基修飾的寡核苷酸,在60μL的ACN中溶解2.3mg的疊氮丁酸NHS酯(DMSO或DMF也是可接受的)。將氨基修飾的寡核苷酸溶解在500μL的0.1M碳酸鹽/碳酸氫鹽緩沖液(pH 9)中。加入12μL的Azidobutyrate NHS酯溶液到溶解的寡核苷酸中,并在室溫下反應2小時并脫鹽。
儲存:干燥,-10至-30°C
稀釋劑:無水ACN,DMSO或DMF溶液
穩定性:2-3天
質量控制: NMR:95%TLC:純
在這種情況下,在任何合成器上像往常一樣合成寡核苷酸。氨基必須在寡核苷酸的3'或5'端引入。在裂解和脫保護之后,疊氮基可以作為胺的修飾被引入。例如,碳酸鈉/碳酸氫鈉緩沖液(pH8.75)中的寡核苷酸在室溫下與琥珀酰亞胺基-4-疊氮基丁酸酯在DMSO中孵育。然后使用HPLC純化終的寡核苷酸或用乙醇或丁醇沉淀。
參考文獻:
J. Am?;瘜W。
使用Click Chemistry
Langmuir,2010,26(11),pp 8666-8672 DOI:10.1021 / ja070273v Template-Directed Oligonucleotide Strand Ligation,Covalent Intramolecular DNA Circularization and Catenation Using Click Chemistry Langmuir,2010,26(11),pp 8666-8672 DOI: 10.1021 / la904822f
將脂質囊泡共價連接到液體支持的雙層允許觀察DNA介導的囊泡相互作用
Cyandye 產品列表:
貨號 | 品名 | 規格 | 品牌 |
Lot 10002 | Azidobutyrate NHS Ester | 50 mg | cyandye |
Lot 10002 | Azidobutyrate NHS Ester | 100 mg | cyandye |
Lot 10002 | Azidobutyrate NHS Ester | 250 mg | cyandye |
Lot 10007 | Cy3 carboxylic acid | 5 mg | cyandye |
Lot 10007 | Cy3 carboxylic acid | 100 mg | cyandye |
Lot 10006 | Cy5 carboxylic acid | 5 mg | cyandye |
Lot 10012 | Cy5.5 carboxylic acid | 5 mg | cyandye |
Lot 10012 | Cy5.5 carboxylic acid | 25 mg | cyandye |
Lot 10012 | Cy5.5 carboxylic acid | 50 mg | cyandye |
Lot 10012 | Cy5.5 carboxylic acid | 100 mg | cyandye |
Lot 10013 | diSulfo - Cy3 alkyne | 1 mg | cyandye |
Lot 10013 | diSulfo - Cy3 alkyne | 5 mg | cyandye |
Lot 10013 | diSulfo - Cy3 alkyne | 10 mg | cyandye |
Lot 10013 | diSulfo - Cy3 alkyne | 25 mg | cyandye |
Lot 10022 | diSulfo - Cy3 azide | 1 mg | cyandye |
Lot 10022 | diSulfo - Cy3 azide | 5 mg | cyandye |
Lot 10022 | diSulfo - Cy3 azide | 10 mg | cyandye |
Lot 10022 | diSulfo - Cy3 azide | 25 mg | cyandye |
Lot a0015 | diSulfo - Cy3 carboxylic acid | 5 mg | cyandye |
Lot a0015 | diSulfo - Cy3 carboxylic acid | 25 mg | cyandye |
Lot a0015 | diSulfo - Cy3 carboxylic acid | 50 mg | cyandye |
Lot a0015 | diSulfo - Cy3 carboxylic acid | 100 mg | cyandye |
Lot 10017 | diSulfo - Cy3 NHS Ester | 5 mg | cyandye |
Lot 10017 | diSulfo - Cy3 NHS Ester | 25 mg | cyandye |
Lot 10017 | diSulfo - Cy3 NHS Ester | 50 mg | cyandye |
Lot 10017 | diSulfo - Cy3 NHS Ester | 100 mg | cyandye |
Lot 10016 | diSulfo - Cy5 alkyne | 1 mg | cyandye |
Lot 10016 | diSulfo - Cy5 alkyne | 5 mg | cyandye |
Lot 10016 | diSulfo - Cy5 alkyne | 10 mg | cyandye |
Lot 10016 | diSulfo - Cy5 alkyne | 25 mg | cyandye |
Lot 10023 | diSulfo - Cy5 azide | 1 mg | cyandye |
Lot 10023 | diSulfo - Cy5 azide | 5 mg | cyandye |
Lot 10010 | diSulfo - Cy5 carboxylic acid | 5 mg | cyandye |
Lot 10010 | diSulfo - Cy5 carboxylic acid | 100 mg | cyandye |
Lot 10009 | diSulfo - Cy5 Hydrazide | 5 mg | cyandye |
Lot 10009 | diSulfo - Cy5 Hydrazide | 25 mg | cyandye |
Lot 10009 | diSulfo - Cy5 Hydrazide | 50 mg | cyandye |
Lot 10009 | diSulfo - Cy5 Hydrazide | 100 mg | cyandye |
Lot 10014 | diSulfo - Cy5 NHS Ester | 5 mg | cyandye |
Lot 10014 | diSulfo - Cy5 NHS Ester | 25 mg | cyandye |
Lot 10014 | diSulfo - Cy5 NHS Ester | 50 mg | cyandye |
Lot 10014 | diSulfo - Cy5 NHS Ester | 100 mg | cyandye |
Lot 10027 | diSulfo - Cy7 carboxylic acid | 5 mg | cyandye |
Lot a0021 | diSulfo - Cy7 NHS Ester | 5 mg | cyandye |
Lot a0021 | diSulfo - Cy7 NHS Ester | 25 mg | cyandye |
Lot a0021 | diSulfo - Cy7 NHS Ester | 50 mg | cyandye |
Lot a0021 | diSulfo - Cy7 NHS Ester | 100 mg | cyandye |
Lot 10004 | monoSulfo - Cy3 alkyne | 1 mg | cyandye |
Lot 10004 | monoSulfo - Cy3 alkyne | 5 mg | cyandye |
Lot 10004 | monoSulfo - Cy3 alkyne | 10 mg | cyandye |
Lot 10004 | monoSulfo - Cy3 alkyne | 100 mg | cyandye |
Lot 10011 | monoSulfo - Cy3 azide | 1 mg | cyandye |
Lot 10011 | monoSulfo - Cy3 azide | 5 mg | cyandye |
Lot 10011 | monoSulfo - Cy3 azide | 10 mg | cyandye |
Lot 10011 | monoSulfo - Cy3 azide | 100 mg | cyandye |
Lot 10025 | monoSulfo - Cy3 carboxylic acid | 5 mg | cyandye |
Lot 10025 | monoSulfo - Cy3 carboxylic acid | 25 mg | cyandye |
Lot 10025 | monoSulfo - Cy3 carboxylic acid | 50 mg | cyandye |
Lot 10025 | monoSulfo - Cy3 carboxylic acid | 100 mg | cyandye |
Lot 10008 | monoSulfo - Cy3 NHS Ester | 5 mg | cyandye |
Lot 10008 | monoSulfo - Cy3 NHS Ester | 100 mg | cyandye |
Lot 10020 | monoSulfo - Cy5 azide | 1 mg | cyandye |
Lot 10020 | monoSulfo - Cy5 azide | 5 mg | cyandye |
Lot 10020 | monoSulfo - Cy5 azide | 10 mg | cyandye |
Lot 10020 | monoSulfo - Cy5 azide | 100 mg | cyandye |
Lot 10026 | monoSulfo - Cy5 carboxylic acid | 5 mg | cyandye |
Lot 10026 | monoSulfo - Cy5 carboxylic acid | 25 mg | cyandye |
Lot 10018 | monoSulfo - Cy5 NHS Ester | 5 mg | cyandye |
Lot 10018 | monoSulfo - Cy5 NHS Ester | 25 mg | cyandye |
Lot a0024 | 3-azidopropylamine | 100 mg | cyandye |
Lot d2501 | 2'-Deoxy-5'-acetyl-5-acetoxymethyluridine | 250 mg | cyandye |
Lot a0001 | 2'-Deoxy-5-hydroxymethyluridine | 250 mg | cyandye |
Lot a0001 | 2'-Deoxy-5-hydroxymethyluridine | 500 mg | cyandye |
Lot a0029 | 4-hydrazinobenzene-1,3-disulfonic acid | 500 mg | cyandye |
Lot a0028 | 5-(hydroxymethyl)-1-[(6aR,8R,9aS)-2,2,4,4-tetraisopropyltetrahydro-6H-furo[3,2-f][1,3,5,2,4]trioxadisilocin-8-yl]pyrimidine-2,4(1H,3H)-dione | 50 mg | cyandye |
Lot a0019 | 2,3,6,7-tetrahydro-1H,5H-pyrido[3,2,1-ij]quinolin-9-ol | 500 mg | cyandye |
Lot a0003 | 2,3,4,6-tetra-O-benzoyl-1-O-(2,2,2-trichloroethanimidoyl)-α-D-glucopyranose | 50 mg | cyandye |
Lot a0003 | 2,3,4,6-tetra-O-benzoyl-1-O-(2,2,2-trichloroethanimidoyl)-α-D-glucopyranose | 100 mg | cyandye |
Lot a0003 | 2,3,4,6-tetra-O-benzoyl-1-O-(2,2,2-trichloroethanimidoyl)-α-D-glucopyranose | 250 mg | cyandye |
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